Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2574123..2574685 Replicon chromosome
Accession NZ_AP022222
Organism Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag H7R20_RS12270 Protein ID WP_000605676.1
Coordinates 2574123..2574401 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H7R20_RS12275 Protein ID WP_000781370.1
Coordinates 2574401..2574685 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R20_RS12245 2569709..2570140 - 432 WP_000152305.1 peroxiredoxin OsmC -
H7R20_RS12250 2570485..2570700 + 216 WP_000495766.1 biofilm-dependent modulation protein -
H7R20_RS12255 2570802..2570939 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H7R20_RS12260 2571096..2572793 + 1698 WP_000433464.1 malate dehydrogenase -
H7R20_RS12265 2572927..2573937 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
H7R20_RS12270 2574123..2574401 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R20_RS12275 2574401..2574685 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
H7R20_RS12280 2574913..2575566 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
H7R20_RS12285 2575559..2576443 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
H7R20_RS12290 2576456..2579503 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T35030 WP_000605676.1 NZ_AP022222:2574123-2574401 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T35030 NZ_AP022222:2574123-2574401 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT35030 WP_000781370.1 NZ_AP022222:2574401-2574685 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT35030 NZ_AP022222:2574401-2574685 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References