Detailed information of TA system TA035013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4808617..4809219
• Replicon: chromosome
• Accession: NZ_AP022221
• Organism: Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7K50_RS22740
• Protein ID: WP_000897305.1
• Coordinates: 4808908..4809219 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7K50_RS22735
• Protein ID: WP_000356395.1
• Coordinates: 4808617..4808907 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K50_RS22700	4804241..4805143	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7K50_RS22705	4805140..4805775	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7K50_RS22710	4805772..4806701	+	930	WP_182645958.1	formate dehydrogenase accessory protein FdhE	-
H7K50_RS22715	4806883..4807125	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
H7K50_RS22720	4807344..4807562	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H7K50_RS22725	4807981..4808259	-	279	WP_001315112.1	hypothetical protein	-
H7K50_RS22730	4808311..4808532	-	222	WP_001550354.1	hypothetical protein	-
H7K50_RS22735	4808617..4808907	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
H7K50_RS22740	4808908..4809219	-	312	WP_000897305.1	hypothetical protein	Toxin
H7K50_RS22745	4809448..4810356	+	909	WP_001550353.1	alpha/beta hydrolase	-
H7K50_RS22750	4810524..4812338	-	1815	WP_001550352.1	hypothetical protein	-
H7K50_RS22755	4812754..4813695	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7K50_RS22760	4813740..4814177	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T35013 WP_000897305.1 NZ_AP022221:c4809219-4808908 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T35013 NZ_AP022221:c4809219-4808908 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT35013 WP_000356395.1 NZ_AP022221:c4808907-4808617 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT35013 NZ_AP022221:c4808907-4808617 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7