Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3887455..3888149 Replicon chromosome
Accession NZ_AP022221
Organism Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag H7K50_RS18430 Protein ID WP_001263491.1
Coordinates 3887455..3887853 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag H7K50_RS18435 Protein ID WP_000554755.1
Coordinates 3887856..3888149 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7K50_RS18400 3882624..3883868 - 1245 WP_000189541.1 esterase FrsA -
- 3883284..3883364 - 81 NuclAT_10 - -
- 3883284..3883364 - 81 NuclAT_10 - -
- 3883284..3883364 - 81 NuclAT_10 - -
- 3883284..3883364 - 81 NuclAT_10 - -
H7K50_RS18405 3883960..3884418 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H7K50_RS18410 3884679..3886136 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
H7K50_RS23690 3886193..3886545 - 353 Protein_3601 peptide chain release factor H -
H7K50_RS18420 3886541..3886747 - 207 Protein_3602 RtcB family protein -
H7K50_RS18425 3886993..3887445 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
H7K50_RS18430 3887455..3887853 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H7K50_RS18435 3887856..3888149 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H7K50_RS18440 3888201..3889256 - 1056 WP_001550655.1 DNA polymerase IV -
H7K50_RS18445 3889327..3890112 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
H7K50_RS18450 3890084..3891796 + 1713 Protein_3608 flagellar biosynthesis protein FlhA -
H7K50_RS18455 3891901..3892179 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H7K50_RS18460 3892172..3892528 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3877391..3888149 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T35007 WP_001263491.1 NZ_AP022221:c3887853-3887455 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T35007 NZ_AP022221:c3887853-3887455 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT35007 WP_000554755.1 NZ_AP022221:c3888149-3887856 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT35007 NZ_AP022221:c3888149-3887856 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References