34991

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1950421..1950923 Replicon chromosome
Accession NZ_AP022221
Organism Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7K50_RS09160 Protein ID WP_000767829.1
Coordinates 1950669..1950923 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7K50_RS09155 Protein ID WP_001259255.1
Coordinates 1950421..1950672 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7K50_RS09130 1945599..1946666 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7K50_RS09135 1946666..1947736 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
H7K50_RS09140 1947733..1949037 - 1305 WP_039267630.1 histidinol dehydrogenase -
H7K50_RS09145 1949043..1949942 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
H7K50_RS09150 1950088..1950138 - 51 WP_071593552.1 his operon leader peptide -
H7K50_RS09155 1950421..1950672 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7K50_RS09160 1950669..1950923 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7K50_RS09165 1951006..1951830 + 825 WP_000754737.1 SDR family oxidoreductase -
H7K50_RS09170 1951876..1952805 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7K50_RS09175 1953020..1953082 + 63 WP_010723108.1 membrane protein YoeI -
H7K50_RS09180 1953072..1954430 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7K50_RS09185 1954609..1955667 + 1059 WP_023568741.1 transport protein YeeE -
H7K50_RS09190 1955681..1955908 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34991 WP_000767829.1 NZ_AP022221:1950669-1950923 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34991 NZ_AP022221:1950669-1950923 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34991 WP_001259255.1 NZ_AP022221:1950421-1950672 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34991 NZ_AP022221:1950421-1950672 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References