Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1950421..1950923 Replicon chromosome
Accession NZ_AP022221
Organism Escherichia coli strain WP8-S17-ESBL-08

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7K50_RS09160 Protein ID WP_000767829.1
Coordinates 1950669..1950923 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7K50_RS09155 Protein ID WP_001259255.1
Coordinates 1950421..1950672 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7K50_RS09130 1945599..1946666 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7K50_RS09135 1946666..1947736 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
H7K50_RS09140 1947733..1949037 - 1305 WP_039267630.1 histidinol dehydrogenase -
H7K50_RS09145 1949043..1949942 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
H7K50_RS09150 1950088..1950138 - 51 WP_071593552.1 his operon leader peptide -
H7K50_RS09155 1950421..1950672 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7K50_RS09160 1950669..1950923 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7K50_RS09165 1951006..1951830 + 825 WP_000754737.1 SDR family oxidoreductase -
H7K50_RS09170 1951876..1952805 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7K50_RS09175 1953020..1953082 + 63 WP_010723108.1 membrane protein YoeI -
H7K50_RS09180 1953072..1954430 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7K50_RS09185 1954609..1955667 + 1059 WP_023568741.1 transport protein YeeE -
H7K50_RS09190 1955681..1955908 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34991 WP_000767829.1 NZ_AP022221:1950669-1950923 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34991 NZ_AP022221:1950669-1950923 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34991 WP_001259255.1 NZ_AP022221:1950421-1950672 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34991 NZ_AP022221:1950421-1950672 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References