Detailed information of TA system TA034982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 57704..58415
• Replicon: plasmid pWP8-S17-ESBL-05_2
• Accession: NZ_AP022217
• Organism: Escherichia coli strain WP8-S17-ESBL-05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: H7R13_RS23685
• Protein ID: WP_000162415.1
• Coordinates: 57704..58006 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R13_RS23690
• Protein ID: WP_000806445.1
• Coordinates: 58077..58415 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R13_RS23655	52723..53373	-	651	WP_032153356.1	hypothetical protein	-
H7R13_RS23660	53387..54292	-	906	WP_001258014.1	recombination-associated protein RdgC	-
H7R13_RS23665	54352..55005	-	654	WP_000828873.1	hypothetical protein	-
H7R13_RS23670	55002..55982	-	981	WP_000046499.1	hypothetical protein	-
H7R13_RS23675	55986..56753	-	768	WP_000203290.1	hypothetical protein	-
H7R13_RS23680	56750..57541	-	792	WP_000532305.1	hypothetical protein	-
H7R13_RS23685	57704..58006	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R13_RS23690	58077..58415	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
H7R13_RS23695	58472..58672	+	201	WP_001022421.1	hypothetical protein	-
H7R13_RS23700	58694..59422	+	729	WP_000986754.1	hypothetical protein	-
H7R13_RS23705	59456..60649	-	1194	WP_001112633.1	hypothetical protein	-
H7R13_RS23710	60642..60971	-	330	WP_000542383.1	hypothetical protein	-
H7R13_RS23715	61300..61953	-	654	WP_000410952.1	hypothetical protein	-
H7R13_RS23720	62221..63120	+	900	WP_000651416.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92332	92332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T34982 WP_000162415.1 NZ_AP022217:57704-58006 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T34982 NZ_AP022217:57704-58006 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT34982 WP_000806445.1 NZ_AP022217:58077-58415 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT34982 NZ_AP022217:58077-58415 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3