Detailed information of TA system TA034976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4608537..4609139
• Replicon: chromosome
• Accession: NZ_AP022215
• Organism: Escherichia coli strain WP8-S17-ESBL-05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7R13_RS21895
• Protein ID: WP_000897305.1
• Coordinates: 4608828..4609139 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R13_RS21890
• Protein ID: WP_000356397.1
• Coordinates: 4608537..4608827 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R13_RS21865	4604463..4605365	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7R13_RS21870	4605362..4605997	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7R13_RS21875	4605994..4606923	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H7R13_RS21880	4607253..4607495	-	243	WP_001086388.1	hypothetical protein	-
H7R13_RS21885	4607714..4607932	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H7R13_RS21890	4608537..4608827	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7R13_RS21895	4608828..4609139	-	312	WP_000897305.1	hypothetical protein	Toxin
H7R13_RS21900	4609368..4610276	+	909	WP_001162704.1	alpha/beta hydrolase	-
H7R13_RS21905	4610340..4611281	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R13_RS21910	4611326..4611763	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R13_RS21915	4611760..4612632	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H7R13_RS21920	4612626..4613225	-	600	WP_001315111.1	glucose-1-phosphatase	-
H7R13_RS21925	4613324..4614109	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T34976 WP_000897305.1 NZ_AP022215:c4609139-4608828 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T34976 NZ_AP022215:c4609139-4608828 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT34976 WP_000356397.1 NZ_AP022215:c4608827-4608537 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT34976 NZ_AP022215:c4608827-4608537 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7