Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1776570..1777072 Replicon chromosome
Accession NZ_AP022215
Organism Escherichia coli strain WP8-S17-ESBL-05

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R13_RS08315 Protein ID WP_000767829.1
Coordinates 1776818..1777072 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R13_RS08310 Protein ID WP_001259255.1
Coordinates 1776570..1776821 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R13_RS08285 1771748..1772815 - 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R13_RS08290 1772815..1773885 - 1071 WP_053292574.1 histidinol-phosphate transaminase -
H7R13_RS08295 1773882..1775186 - 1305 WP_000009594.1 histidinol dehydrogenase -
H7R13_RS08300 1775192..1776091 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R13_RS08305 1776237..1776287 - 51 WP_001364200.1 his operon leader peptide -
H7R13_RS08310 1776570..1776821 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R13_RS08315 1776818..1777072 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R13_RS08320 1777155..1777979 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R13_RS08325 1778025..1778954 + 930 WP_053292573.1 LysR family transcriptional regulator -
H7R13_RS08330 1779169..1779231 + 63 WP_010723108.1 membrane protein YoeI -
H7R13_RS08335 1779221..1780579 + 1359 WP_024250698.1 putrescine/proton symporter PlaP -
H7R13_RS08340 1780758..1781816 + 1059 WP_000492311.1 transport protein YeeE -
H7R13_RS08345 1781830..1782057 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34959 WP_000767829.1 NZ_AP022215:1776818-1777072 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34959 NZ_AP022215:1776818-1777072 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34959 WP_001259255.1 NZ_AP022215:1776570-1776821 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34959 NZ_AP022215:1776570-1776821 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References