Detailed information of TA system TA034943

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110133..110403
• Replicon: plasmid pWP7-S18-ESBL-09_1
• Accession: NZ_AP022208
• Organism: Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R37_RS25675
• Protein ID: WP_001312861.1
• Coordinates: 110245..110403 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	110133..110196 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R37_RS25640	105146..105601	-	456	WP_059337654.1	hypothetical protein	-
H7R37_RS25645	105903..106430	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H7R37_RS25650	106488..106721	+	234	WP_000006003.1	DUF905 family protein	-
H7R37_RS25655	106782..108746	+	1965	WP_025746229.1	ParB/RepB/Spo0J family partition protein	-
H7R37_RS25660	108815..109249	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H7R37_RS25665	109246..109965	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	109977..110201	+	225	NuclAT_0	-	-
-	109977..110201	+	225	NuclAT_0	-	-
-	109977..110201	+	225	NuclAT_0	-	-
-	109977..110201	+	225	NuclAT_0	-	-
H7R37_RS25670	109986..110165	-	180	WP_001309233.1	hypothetical protein	-
-	110133..110196	-	64	-	-	Antitoxin
H7R37_RS25675	110245..110403	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R37_RS25680	110704..110908	-	205	Protein_144	pilus protein	-
H7R37_RS25685	111300..111596	+	297	WP_001272251.1	hypothetical protein	-
H7R37_RS25690	111707..112528	+	822	WP_001234445.1	DUF945 domain-containing protein	-
H7R37_RS25695	112825..113415	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
H7R37_RS25700	113748..114131	+	384	WP_001151529.1	relaxosome protein TraM	-
H7R37_RS25705	114324..114971	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
H7R37_RS25710	115079..115318	+	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	senB	1..214586	214586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T34943 WP_001312861.1 NZ_AP022208:110245-110403 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34943 NZ_AP022208:110245-110403 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT34943 NZ_AP022208:c110196-110133 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin