Detailed information of TA system TA034940

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83660..83913
• Replicon: plasmid pWP7-S18-ESBL-09_1
• Accession: NZ_AP022208
• Organism: Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H7R37_RS25490
• Protein ID: WP_001312851.1
• Coordinates: 83764..83913 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	83660..83719 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R37_RS25455	78838..79299	+	462	WP_001233838.1	thermonuclease family protein	-
H7R37_RS25460	79345..79554	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
H7R37_RS25465	79592..80182	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
H7R37_RS25470	80369..81940	-	1572	WP_001526014.1	IS66 family transposase	-
H7R37_RS25475	81960..82307	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R37_RS25480	82307..82984	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
H7R37_RS25485	83044..83517	+	474	WP_016240489.1	hypothetical protein	-
-	83660..83719	-	60	NuclAT_2	-	Antitoxin
-	83660..83719	-	60	NuclAT_2	-	Antitoxin
-	83660..83719	-	60	NuclAT_2	-	Antitoxin
-	83660..83719	-	60	NuclAT_2	-	Antitoxin
H7R37_RS25490	83764..83913	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R37_RS25495	84198..84446	+	249	WP_000083839.1	replication regulatory protein RepA	-
H7R37_RS25500	84691..84765	+	75	WP_001365705.1	RepA leader peptide Tap	-
H7R37_RS25505	84758..85615	+	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
H7R37_RS25510	86686..86949	+	264	WP_001089473.1	hypothetical protein	-
H7R37_RS25515	86939..87238	+	300	WP_182916814.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H7R37_RS25520	87274..87939	-	666	WP_001535733.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	senB	1..214586	214586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T34940 WP_001312851.1 NZ_AP022208:83764-83913 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T34940 NZ_AP022208:83764-83913 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT34940 NZ_AP022208:c83719-83660 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin