Detailed information of TA system TA034936

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39363..39785
• Replicon: plasmid pWP7-S18-ESBL-09_1
• Accession: NZ_AP022208
• Organism: Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: H7R37_RS25225
• Protein ID: WP_001302184.1
• Coordinates: 39627..39785 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39363..39562 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R37_RS25195	34558..35010	-	453	WP_024185774.1	hypothetical protein	-
H7R37_RS25200	35312..35839	+	528	WP_000290833.1	single-stranded DNA-binding protein	-
H7R37_RS25205	35896..36129	+	234	WP_000006018.1	DUF905 domain-containing protein	-
H7R37_RS25210	36188..38146	+	1959	WP_001145501.1	ParB/RepB/Spo0J family partition protein	-
H7R37_RS25215	38201..38635	+	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
H7R37_RS25220	38632..39351	+	720	WP_001276230.1	plasmid SOS inhibition protein A	-
-	39363..39562	+	200	NuclAT_1	-	Antitoxin
-	39363..39562	+	200	NuclAT_1	-	Antitoxin
-	39363..39562	+	200	NuclAT_1	-	Antitoxin
-	39363..39562	+	200	NuclAT_1	-	Antitoxin
H7R37_RS25225	39627..39785	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R37_RS25230	40701..40988	+	288	WP_000107526.1	hypothetical protein	-
H7R37_RS25235	41107..41928	+	822	WP_001234489.1	DUF945 domain-containing protein	-
H7R37_RS25240	42224..42826	-	603	WP_001323326.1	transglycosylase SLT domain-containing protein	-
H7R37_RS25245	43157..43540	+	384	WP_001151562.1	relaxosome protein TraM	-
H7R37_RS25250	43734..44420	+	687	WP_000332487.1	PAS domain-containing protein	-
H7R37_RS25255	44514..44741	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	senB	1..214586	214586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T34936 WP_001302184.1 NZ_AP022208:39627-39785 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34936 NZ_AP022208:39627-39785 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 200 bp

>AT34936 NZ_AP022208:39363-39562 [Escherichia coli]
TCACAACGGATTGCCCGTAAACAGCCTGAATGAGCGAGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATAT
GGGCTATGATGTGCCCGGCGCTTGAGGTTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGG
ACAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin