Detailed information of TA system TA034935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5001546..5002148
• Replicon: chromosome
• Accession: NZ_AP022207
• Organism: Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7R37_RS24010
• Protein ID: WP_000897305.1
• Coordinates: 5001837..5002148 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R37_RS24005
• Protein ID: WP_000356395.1
• Coordinates: 5001546..5001836 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R37_RS23975	4997169..4998071	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7R37_RS23980	4998068..4998703	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7R37_RS23985	4998700..4999629	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H7R37_RS23990	4999811..5000053	-	243	WP_021523315.1	hypothetical protein	-
H7R37_RS23995	5000272..5000490	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H7R37_RS24000	5000909..5001187	-	279	WP_001306650.1	hypothetical protein	-
H7R37_RS24005	5001546..5001836	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
H7R37_RS24010	5001837..5002148	-	312	WP_000897305.1	hypothetical protein	Toxin
H7R37_RS24015	5002377..5003285	+	909	WP_001306651.1	alpha/beta hydrolase	-
H7R37_RS24020	5003454..5005268	-	1815	WP_020233236.1	hypothetical protein	-
H7R37_RS24025	5005684..5006625	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R37_RS24030	5006670..5007107	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T34935 WP_000897305.1 NZ_AP022207:c5002148-5001837 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T34935 NZ_AP022207:c5002148-5001837 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT34935 WP_000356395.1 NZ_AP022207:c5001836-5001546 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT34935 NZ_AP022207:c5001836-5001546 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7