Detailed information of TA system TA034929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4111454..4112148
• Replicon: chromosome
• Accession: NZ_AP022207
• Organism: Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: H7R37_RS19860
• Protein ID: WP_001521903.1
• Coordinates: 4111454..4111852 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R37_RS19865
• Protein ID: WP_000554758.1
• Coordinates: 4111855..4112148 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R37_RS19830	4106624..4107868	-	1245	WP_000189541.1	esterase FrsA	-
-	4107284..4107364	-	81	NuclAT_11	-	-
-	4107284..4107364	-	81	NuclAT_11	-	-
-	4107284..4107364	-	81	NuclAT_11	-	-
-	4107284..4107364	-	81	NuclAT_11	-	-
H7R37_RS19835	4107960..4108418	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R37_RS19840	4108679..4110136	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
H7R37_RS26360	4110193..4110545	-	353	Protein_3883	peptide chain release factor H	-
H7R37_RS19850	4110541..4110747	-	207	Protein_3884	RtcB family protein	-
H7R37_RS19855	4110992..4111444	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
H7R37_RS19860	4111454..4111852	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R37_RS19865	4111855..4112148	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R37_RS19870	4112200..4113255	-	1056	WP_001226177.1	DNA polymerase IV	-
H7R37_RS19875	4113326..4114111	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
H7R37_RS19880	4114083..4115795	+	1713	Protein_3890	flagellar biosynthesis protein FlhA	-
H7R37_RS19885	4115874..4116227	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
H7R37_RS19890	4116284..4117033	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4101391..4112148	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T34929 WP_001521903.1 NZ_AP022207:c4111852-4111454 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T34929 NZ_AP022207:c4111852-4111454 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT34929 WP_000554758.1 NZ_AP022207:c4112148-4111855 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT34929 NZ_AP022207:c4112148-4111855 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3