Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1977943..1978445 Replicon chromosome
Accession NZ_AP022207
Organism Escherichia coli strain WP7-S18-ESBL-09

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R37_RS09245 Protein ID WP_000767829.1
Coordinates 1978191..1978445 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R37_RS09240 Protein ID WP_001259255.1
Coordinates 1977943..1978194 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R37_RS09215 1973120..1974187 - 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R37_RS09220 1974187..1975257 - 1071 WP_001099214.1 histidinol-phosphate transaminase -
H7R37_RS09225 1975254..1976558 - 1305 WP_001396164.1 histidinol dehydrogenase -
H7R37_RS09230 1976564..1977463 - 900 WP_000566782.1 ATP phosphoribosyltransferase -
H7R37_RS09235 1977609..1977659 - 51 WP_001364200.1 his operon leader peptide -
H7R37_RS09240 1977943..1978194 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R37_RS09245 1978191..1978445 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R37_RS09250 1978528..1979352 + 825 WP_000754754.1 SDR family oxidoreductase -
H7R37_RS09255 1979398..1980327 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R37_RS09260 1980542..1980604 + 63 WP_010723108.1 membrane protein YoeI -
H7R37_RS09265 1980594..1981952 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R37_RS09270 1982131..1983189 + 1059 WP_000492305.1 transport protein YeeE -
H7R37_RS09275 1983203..1983430 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34914 WP_000767829.1 NZ_AP022207:1978191-1978445 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34914 NZ_AP022207:1978191-1978445 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34914 WP_001259255.1 NZ_AP022207:1977943-1978194 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34914 NZ_AP022207:1977943-1978194 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References