Detailed information of TA system TA034877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3688978..3689672
• Replicon: chromosome
• Accession: NZ_AP022177
• Organism: Escherichia coli strain WP7-S17-ESBL-04

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H7R11_RS17795
• Protein ID: WP_001263489.1
• Coordinates: 3688978..3689376 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R11_RS17800
• Protein ID: WP_000554758.1
• Coordinates: 3689379..3689672 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3684566..3684646	-	81	NuclAT_12	-	-
-	3684566..3684646	-	81	NuclAT_12	-	-
-	3684566..3684646	-	81	NuclAT_12	-	-
-	3684566..3684646	-	81	NuclAT_12	-	-
H7R11_RS17770	3685242..3685700	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R11_RS17775	3685961..3687418	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H7R11_RS17780	3687475..3687996	-	522	Protein_3477	peptide chain release factor H	-
H7R11_RS17785	3687992..3688198	-	207	Protein_3478	RtcB family protein	-
H7R11_RS17790	3688516..3688968	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H7R11_RS17795	3688978..3689376	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R11_RS17800	3689379..3689672	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R11_RS17805	3689724..3690779	-	1056	WP_001226164.1	DNA polymerase IV	-
H7R11_RS17810	3690850..3691635	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H7R11_RS17815	3691607..3693319	+	1713	Protein_3484	flagellar biosynthesis protein FlhA	-
H7R11_RS17820	3693543..3694040	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3653767..3694842	41075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T34877 WP_001263489.1 NZ_AP022177:c3689376-3688978 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T34877 NZ_AP022177:c3689376-3688978 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT34877 WP_000554758.1 NZ_AP022177:c3689672-3689379 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT34877 NZ_AP022177:c3689672-3689379 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3