Detailed information of TA system TA034845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4035927..4036577
• Replicon: chromosome
• Accession: NZ_AP022173
• Organism: Escherichia coli strain WP7-S17-ESBL-01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R26_RS19670
• Protein ID: WP_000263532.1
• Coordinates: 4036236..4036577 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: H7R26_RS19665
• Protein ID: WP_000212552.1
• Coordinates: 4035927..4036226 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R26_RS19640	4031375..4031686	-	312	WP_000415004.1	hypothetical protein	-
H7R26_RS19645	4031691..4032083	-	393	WP_000285322.1	flagellar export chaperone FliS	-
H7R26_RS19650	4032106..4033422	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
H7R26_RS19655	4033627..4034541	-	915	WP_000949083.1	lateral flagellin LafA	-
H7R26_RS19660	4035027..4035869	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
H7R26_RS19665	4035927..4036226	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
H7R26_RS19670	4036236..4036577	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R26_RS19675	4036653..4037630	-	978	WP_001195938.1	hypothetical protein	-
H7R26_RS19680	4037647..4038576	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
H7R26_RS19685	4038591..4039967	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
H7R26_RS19690	4040143..4040442	-	300	WP_000867281.1	rod-binding protein	-
H7R26_RS19695	4040442..4041542	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T34845 WP_000263532.1 NZ_AP022173:c4036577-4036236 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T34845 NZ_AP022173:c4036577-4036236 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT34845 WP_000212552.1 NZ_AP022173:c4036226-4035927 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT34845 NZ_AP022173:c4036226-4035927 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7