Detailed information of TA system TA034817

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134383..134806
• Replicon: plasmid pWP5-S18-ESBL-09_1
• Accession: NZ_AP022172
• Organism: Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R27_RS25075
• Protein ID: WP_001312861.1
• Coordinates: 134383..134541 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	134585..134806 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R27_RS25045	129450..129689	-	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-
H7R27_RS25050	129797..130444	-	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
H7R27_RS25055	130636..131019	-	384	WP_001151529.1	relaxosome protein TraM	-
H7R27_RS25060	131295..131942	+	648	Protein_166	transglycosylase SLT domain-containing protein	-
H7R27_RS25065	132239..133060	-	822	WP_021549731.1	DUF945 domain-containing protein	-
H7R27_RS25070	133171..133467	-	297	WP_001272251.1	hypothetical protein	-
H7R27_RS25075	134383..134541	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	134585..134806	+	222	-	-	Antitoxin
-	134585..134809	-	225	NuclAT_0	-	-
-	134585..134809	-	225	NuclAT_0	-	-
-	134585..134809	-	225	NuclAT_0	-	-
-	134585..134809	-	225	NuclAT_0	-	-
H7R27_RS25085	134821..135540	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
H7R27_RS25090	135537..135971	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H7R27_RS25095	136040..138004	-	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
H7R27_RS25100	138065..138298	-	234	WP_000006003.1	DUF905 family protein	-
H7R27_RS25105	138356..138883	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
H7R27_RS25110	139185..139634	+	450	WP_024248742.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..187669	187669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T34817 WP_001312861.1 NZ_AP022172:c134541-134383 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34817 NZ_AP022172:c134541-134383 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 222 bp

>AT34817 NZ_AP022172:134585-134806 [Escherichia coli]
ATGTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTTTCTGCCACACA
ACACGGTAACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGC
TGACCACACTCACTTTTCCTGAAAATAACCCGCTCATTCAGGCTGTTTACGGGTAATCTGTG

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin