Detailed information of TA system TA034813

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65444..65866
• Replicon: plasmid pWP5-S18-ESBL-09_1
• Accession: NZ_AP022172
• Organism: Escherichia coli strain WP5-S18-ESBL-09

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: H7R27_RS24640
• Protein ID: WP_001302184.1
• Coordinates: 65444..65602 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65667..65866 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R27_RS24610	60488..60715	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
H7R27_RS24615	60809..61495	-	687	WP_000332487.1	PAS domain-containing protein	-
H7R27_RS24620	61689..62072	-	384	WP_001151562.1	relaxosome protein TraM	-
H7R27_RS24625	62403..63005	+	603	WP_001323326.1	transglycosylase SLT domain-containing protein	-
H7R27_RS24630	63301..64122	-	822	WP_001234489.1	DUF945 domain-containing protein	-
H7R27_RS24635	64241..64528	-	288	WP_000107526.1	hypothetical protein	-
H7R27_RS24640	65444..65602	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	65667..65866	-	200	NuclAT_1	-	Antitoxin
-	65667..65866	-	200	NuclAT_1	-	Antitoxin
-	65667..65866	-	200	NuclAT_1	-	Antitoxin
-	65667..65866	-	200	NuclAT_1	-	Antitoxin
H7R27_RS24645	65878..66597	-	720	WP_001276230.1	plasmid SOS inhibition protein A	-
H7R27_RS24650	66594..67028	-	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
H7R27_RS24655	67083..69041	-	1959	WP_001145501.1	ParB/RepB/Spo0J family partition protein	-
H7R27_RS24660	69100..69333	-	234	WP_000006018.1	DUF905 domain-containing protein	-
H7R27_RS24665	69390..69917	-	528	WP_000290833.1	single-stranded DNA-binding protein	-
H7R27_RS24670	70219..70677	+	459	WP_011478091.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..187669	187669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T34813 WP_001302184.1 NZ_AP022172:c65602-65444 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34813 NZ_AP022172:c65602-65444 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 200 bp

>AT34813 NZ_AP022172:c65866-65667 [Escherichia coli]
TCACAACGGATTGCCCGTAAACAGCCTGAATGAGCGAGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATAT
GGGCTATGATGTGCCCGGCGCTTGAGGTTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGG
ACAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin