Detailed information of TA system TA034785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 42376..43022
• Replicon: plasmid pWP5-S18-ESBL-08_3
• Accession: NZ_AP022164
• Organism: Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R36_RS26520
• Protein ID: WP_001673378.1
• Coordinates: 42376..42723 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1V3UZT0
• Locus tag: H7R36_RS26525
• Protein ID: WP_001259436.1
• Coordinates: 42723..43022 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R36_RS26495	38323..38940	+	618	WP_001523080.1	tail fiber assembly protein	-
H7R36_RS26500	38904..39449	-	546	WP_001704996.1	transferase	-
H7R36_RS26505	40078..40914	+	837	WP_001139597.1	hypothetical protein	-
H7R36_RS26510	41557..41844	+	288	WP_000356589.1	hypothetical protein	-
H7R36_RS26515	41868..42131	+	264	WP_000424604.1	hypothetical protein	-
H7R36_RS26520	42376..42723	+	348	WP_001673378.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R36_RS26525	42723..43022	+	300	WP_001259436.1	XRE family transcriptional regulator	Antitoxin
H7R36_RS26530	43186..43566	+	381	WP_001523084.1	hypothetical protein	-
H7R36_RS26535	43670..44095	-	426	WP_016240784.1	hypothetical protein	-
H7R36_RS26540	44197..44469	+	273	WP_000148348.1	helix-turn-helix domain-containing protein	-
H7R36_RS26545	45081..45269	+	189	WP_004105254.1	hypothetical protein	-
H7R36_RS26550	45280..46425	-	1146	WP_112034448.1	ORF6N domain-containing protein	-
H7R36_RS26555	46422..46613	-	192	WP_000183351.1	hypothetical protein	-
H7R36_RS26560	46691..46864	-	174	WP_157838146.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..47454	47454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13544.52 Da
• Isoelectric Point: 9.3634

>T34785 WP_001673378.1 NZ_AP022164:42376-42723 [Escherichia coli]
MWTVLFSQRFDDWLNEQEEALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T34785 NZ_AP022164:42376-42723 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGAGGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAATCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11074.92 Da
• Isoelectric Point: 5.2090

>AT34785 WP_001259436.1 NZ_AP022164:42723-43022 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT34785 NZ_AP022164:42723-43022 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V3UZT0