Detailed information of TA system TA034781

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 117297..117566
• Replicon: plasmid pWP5-S18-ESBL-08_1
• Accession: NZ_AP022162
• Organism: Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R36_RS25550
• Protein ID: WP_001312861.1
• Coordinates: 117408..117566 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	117297..117362 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R36_RS25530	112518..114476	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
H7R36_RS25535	114531..114965	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
H7R36_RS25540	114962..115681	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	115693..115795	+	103	NuclAT_1	-	-
-	115693..115795	+	103	NuclAT_1	-	-
-	115693..115795	+	103	NuclAT_1	-	-
-	115693..115795	+	103	NuclAT_1	-	-
H7R36_RS25545	115841..117210	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	117237..117364	+	128	NuclAT_0	-	-
-	117237..117364	+	128	NuclAT_0	-	-
-	117237..117364	+	128	NuclAT_0	-	-
-	117237..117364	+	128	NuclAT_0	-	-
-	117297..117362	+	66	-	-	Antitoxin
H7R36_RS25550	117408..117566	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R36_RS25555	117861..118016	+	156	WP_001380190.1	hypothetical protein	-
H7R36_RS25560	118341..119096	-	756	WP_001282653.1	IS21-like element ISSso4 family helper ATPase IstB	-
H7R36_RS25565	119113..120648	-	1536	WP_000447015.1	IS21 family transposase	-
H7R36_RS25570	120902..121108	+	207	WP_000275859.1	hypothetical protein	-
H7R36_RS25575	121133..121420	+	288	WP_000107535.1	hypothetical protein	-
H7R36_RS25580	121539..122360	+	822	WP_001234469.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..147186	147186
	-	inside	IScluster/Tn	-	-	115841..120648	4807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T34781 WP_001312861.1 NZ_AP022162:117408-117566 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34781 NZ_AP022162:117408-117566 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT34781 NZ_AP022162:117297-117362 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin