Detailed information of TA system TA034773

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53..292
• Replicon: plasmid pWP5-S18-ESBL-08_1
• Accession: NZ_AP022162
• Organism: Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: H7R36_RS24850
• Protein ID: WP_023144756.1
• Coordinates: 158..292 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53..113 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	53..113	-	61	NuclAT_2	-	Antitoxin
-	53..113	-	61	NuclAT_2	-	Antitoxin
-	53..113	-	61	NuclAT_2	-	Antitoxin
-	53..113	-	61	NuclAT_2	-	Antitoxin
H7R36_RS24850	158..292	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R36_RS24855	589..699	+	111	Protein_2	replication protein	-
H7R36_RS24860	768..1547	-	780	WP_001323403.1	IS21-like element IS100kyp family helper ATPase IstB	-
H7R36_RS24865	1547..2569	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
H7R36_RS24870	2650..2802	+	153	WP_072258247.1	replication protein A	-
H7R36_RS26745	2908..3042	+	135	Protein_6	protein CopA/IncA	-
H7R36_RS24875	3039..3113	+	75	WP_031943482.1	RepA leader peptide Tap	-
H7R36_RS24880	3106..3963	+	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
H7R36_RS24885	4665..4805	+	141	WP_001333237.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..147186	147186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T34773 WP_023144756.1 NZ_AP022162:158-292 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T34773 NZ_AP022162:158-292 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT34773 NZ_AP022162:c113-53 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin