Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4027717..4028411 Replicon chromosome
Accession NZ_AP022161
Organism Escherichia coli strain WP5-S18-ESBL-08

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag H7R36_RS19320 Protein ID WP_001263491.1
Coordinates 4027717..4028115 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag H7R36_RS19325 Protein ID WP_000554755.1
Coordinates 4028118..4028411 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R36_RS19290 4022979..4024436 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
H7R36_RS19295 4024445..4024726 + 282 WP_022645226.1 hypothetical protein -
H7R36_RS19300 4024743..4025252 - 510 WP_001361775.1 hydrolase -
H7R36_RS19305 4025314..4025928 - 615 WP_022645225.1 peptide chain release factor H -
H7R36_RS19310 4025925..4027064 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
H7R36_RS19315 4027255..4027707 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
H7R36_RS19320 4027717..4028115 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H7R36_RS19325 4028118..4028411 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H7R36_RS19330 4028463..4029518 - 1056 WP_022645222.1 DNA polymerase IV -
H7R36_RS19335 4029589..4030374 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
H7R36_RS19340 4030346..4032058 + 1713 Protein_3793 flagellar biosynthesis protein FlhA -
H7R36_RS19345 4032156..4032929 - 774 WP_022645219.1 C40 family peptidase -
H7R36_RS19350 4033115..4033375 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T34766 WP_001263491.1 NZ_AP022161:c4028115-4027717 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T34766 NZ_AP022161:c4028115-4027717 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT34766 WP_000554755.1 NZ_AP022161:c4028411-4028118 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT34766 NZ_AP022161:c4028411-4028118 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References