Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1919937..1920439 Replicon chromosome
Accession NZ_AP022159
Organism Escherichia coli strain WP5-S18-ESBL-07

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R21_RS09005 Protein ID WP_000767829.1
Coordinates 1920185..1920439 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R21_RS09000 Protein ID WP_001259255.1
Coordinates 1919937..1920188 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R21_RS08975 1915115..1916182 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R21_RS08980 1916182..1917252 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
H7R21_RS08985 1917249..1918553 - 1305 WP_039267630.1 histidinol dehydrogenase -
H7R21_RS08990 1918559..1919458 - 900 WP_182913358.1 ATP phosphoribosyltransferase -
H7R21_RS08995 1919604..1919654 - 51 WP_071593552.1 his operon leader peptide -
H7R21_RS09000 1919937..1920188 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R21_RS09005 1920185..1920439 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R21_RS09010 1920522..1921346 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R21_RS09015 1921392..1922321 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R21_RS09020 1922536..1922598 + 63 WP_010723108.1 membrane protein YoeI -
H7R21_RS09025 1922588..1923946 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R21_RS09030 1924125..1925183 + 1059 WP_023568741.1 transport protein YeeE -
H7R21_RS09035 1925197..1925424 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34721 WP_000767829.1 NZ_AP022159:1920185-1920439 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34721 NZ_AP022159:1920185-1920439 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34721 WP_001259255.1 NZ_AP022159:1919937-1920188 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34721 NZ_AP022159:1919937-1920188 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References