Detailed information of TA system TA034711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 48237..48964
• Replicon: plasmid pWP5-S18-ESBL-05_2
• Accession: NZ_AP022144
• Organism: Klebsiella quasipneumoniae strain WP5-S18-ESBL-05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: H7R80_RS27290
• Protein ID: WP_011251285.1
• Coordinates: 48237..48548 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R80_RS27295
• Protein ID: WP_011251286.1
• Coordinates: 48545..48964 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R80_RS27260	43428..44408	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
H7R80_RS27265	44954..45133	-	180	Protein_56	IS5/IS1182 family transposase	-
H7R80_RS27270	45173..46711	-	1539	WP_017901237.1	IS66 family transposase	-
H7R80_RS27275	46760..47107	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R80_RS27280	47104..47481	-	378	WP_004118218.1	IS66 family insertion sequence hypothetical protein	-
H7R80_RS27285	47574..48032	+	459	Protein_60	DDE-type integrase/transposase/recombinase	-
H7R80_RS27290	48237..48548	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R80_RS27295	48545..48964	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
H7R80_RS27300	49034..49447	+	414	WP_017901337.1	hypothetical protein	-
H7R80_RS27305	49493..50461	-	969	WP_074168518.1	IS5-like element IS903B family transposase	-
H7R80_RS27310	50565..50831	+	267	WP_017900868.1	hypothetical protein	-
H7R80_RS27315	50945..51480	-	536	Protein_66	transposase	-
H7R80_RS27320	52005..53372	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..182573	182573
	-	inside	IScluster/Tn	-	-	43428..50461	7033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T34711 WP_011251285.1 NZ_AP022144:48237-48548 [Klebsiella quasipneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T34711 NZ_AP022144:48237-48548 [Klebsiella quasipneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT34711 WP_011251286.1 NZ_AP022144:48545-48964 [Klebsiella quasipneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT34711 NZ_AP022144:48545-48964 [Klebsiella quasipneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1