Detailed information of TA system TA034655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31815..32545
• Replicon: chromosome
• Accession: NZ_AP022126
• Organism: Enterobacter cloacae strain WP5-S18-CRE-02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J0PHU0
• Locus tag: H7R61_RS00155
• Protein ID: WP_014881928.1
• Coordinates: 31815..32129 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R61_RS00160
• Protein ID: WP_023331687.1
• Coordinates: 32126..32545 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R61_RS00130	27480..28568	+	1089	WP_014881924.1	cellulase family glycosylhydrolase	-
H7R61_RS00135	28471..29655	-	1185	WP_014881925.1	multidrug efflux MFS transporter EmrD	-
H7R61_RS00140	29831..30664	-	834	WP_014881926.1	DMT family transporter	-
H7R61_RS00145	30727..31173	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
H7R61_RS00150	31238..31327	-	90	WP_023331685.1	type I toxin-antitoxin system toxin TisB	-
H7R61_RS00155	31815..32129	+	315	WP_014881928.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R61_RS00160	32126..32545	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
H7R61_RS00165	32695..32793	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
H7R61_RS00170	32900..34588	+	1689	WP_014881930.1	acetolactate synthase large subunit	-
H7R61_RS00175	34592..34879	+	288	WP_010426528.1	acetolactate synthase small subunit	-
H7R61_RS00180	34962..35555	+	594	WP_014881931.1	transcriptional regulator UhpA	-
H7R61_RS00185	35552..37057	+	1506	WP_014881932.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12437.60 Da
• Isoelectric Point: 10.2825

>T34655 WP_014881928.1 NZ_AP022126:31815-32129 [Enterobacter cloacae]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCPTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T34655 NZ_AP022126:31815-32129 [Enterobacter cloacae]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCCCGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT34655 WP_023331687.1 NZ_AP022126:32126-32545 [Enterobacter cloacae]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT34655 NZ_AP022126:32126-32545 [Enterobacter cloacae]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA

Structures

Toxin

• AlphaFold DB: A0A2J0PHU0