Detailed information of TA system TA034651

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59830..60094
• Replicon: plasmid pWP5-W18-ESBL-11_2
• Accession: NZ_AP022122
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: H7R04_RS23885
• Protein ID: WP_001303307.1
• Coordinates: 59942..60094 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	59830..59892 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS23870	55069..57360	-	2292	WP_024255997.1	hypothetical protein	-
H7R04_RS23875	57353..58423	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
H7R04_RS23880	58442..59650	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	59830..59892	-	63	NuclAT_0	-	Antitoxin
-	59830..59892	-	63	NuclAT_0	-	Antitoxin
-	59830..59892	-	63	NuclAT_0	-	Antitoxin
-	59830..59892	-	63	NuclAT_0	-	Antitoxin
H7R04_RS23885	59942..60094	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
H7R04_RS23890	60166..60417	-	252	WP_001291965.1	hypothetical protein	-
-	60804..60855	-	52	NuclAT_1	-	-
-	60804..60855	-	52	NuclAT_1	-	-
-	60804..60855	-	52	NuclAT_1	-	-
-	60804..60855	-	52	NuclAT_1	-	-
H7R04_RS23895	61341..61517	-	177	WP_001054900.1	hypothetical protein	-
H7R04_RS23900	61909..62118	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H7R04_RS23905	62190..62852	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
H7R04_RS23910	62923..65091	-	2169	WP_052974685.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-8	-	1..89476	89476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T34651 WP_001303307.1 NZ_AP022122:59942-60094 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T34651 NZ_AP022122:59942-60094 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT34651 NZ_AP022122:c59892-59830 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin