Detailed information of TA system TA034644

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53733..54154
• Replicon: plasmid pWP5-W18-ESBL-11_1
• Accession: NZ_AP022121
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: H7R04_RS23020
• Protein ID: WP_001302184.1
• Coordinates: 53996..54154 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53733..53931 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS22990	48836..49345	-	510	WP_071600123.1	hypothetical protein	-
H7R04_RS22995	49663..50202	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
H7R04_RS23000	50259..50492	+	234	WP_000005990.1	DUF905 family protein	-
H7R04_RS23005	50558..52516	+	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
H7R04_RS23010	52571..53005	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
H7R04_RS23015	53002..53721	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	53733..53931	+	199	NuclAT_0	-	Antitoxin
-	53733..53931	+	199	NuclAT_0	-	Antitoxin
-	53733..53931	+	199	NuclAT_0	-	Antitoxin
-	53733..53931	+	199	NuclAT_0	-	Antitoxin
H7R04_RS23020	53996..54154	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R04_RS23025	54392..54751	-	360	Protein_56	hypothetical protein	-
H7R04_RS23030	54821..55027	+	207	WP_000547965.1	hypothetical protein	-
H7R04_RS23035	55052..55339	+	288	WP_000107546.1	hypothetical protein	-
H7R04_RS23040	55458..56279	+	822	WP_001234475.1	DUF945 domain-containing protein	-
H7R04_RS23045	56574..57164	-	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
H7R04_RS23050	57516..57899	+	384	WP_001063020.1	relaxosome protein TraM	-
H7R04_RS23055	58091..58738	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
H7R04_RS23060	58874..59089	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..158291	158291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T34644 WP_001302184.1 NZ_AP022121:53996-54154 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34644 NZ_AP022121:53996-54154 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT34644 NZ_AP022121:53733-53931 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin