Detailed information of TA system TA034640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4586117..4586719
• Replicon: chromosome
• Accession: NZ_AP022120
• Organism: Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7R04_RS21755
• Protein ID: WP_000897305.1
• Coordinates: 4586408..4586719 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R04_RS21750
• Protein ID: WP_000356397.1
• Coordinates: 4586117..4586407 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R04_RS21725	4582042..4582944	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7R04_RS21730	4582941..4583576	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7R04_RS21735	4583573..4584502	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H7R04_RS21740	4584832..4585074	-	243	WP_001087409.1	hypothetical protein	-
H7R04_RS21745	4585294..4585512	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
H7R04_RS21750	4586117..4586407	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7R04_RS21755	4586408..4586719	-	312	WP_000897305.1	hypothetical protein	Toxin
H7R04_RS21760	4586948..4587856	+	909	WP_001162704.1	alpha/beta hydrolase	-
H7R04_RS21765	4587920..4588861	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R04_RS21770	4588906..4589343	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R04_RS21775	4589340..4590212	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H7R04_RS21780	4590206..4590805	-	600	WP_001295269.1	glucose-1-phosphatase	-
H7R04_RS21785	4590904..4591689	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T34640 WP_000897305.1 NZ_AP022120:c4586719-4586408 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T34640 NZ_AP022120:c4586719-4586408 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT34640 WP_000356397.1 NZ_AP022120:c4586407-4586117 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT34640 NZ_AP022120:c4586407-4586117 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7