Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1832451..1832953 Replicon chromosome
Accession NZ_AP022120
Organism Escherichia coli strain WP5-W18-ESBL-11

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R04_RS08680 Protein ID WP_000767829.1
Coordinates 1832699..1832953 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R04_RS08675 Protein ID WP_001259255.1
Coordinates 1832451..1832702 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R04_RS08650 1827628..1828695 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R04_RS08655 1828695..1829765 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
H7R04_RS08660 1829762..1831066 - 1305 WP_001446927.1 histidinol dehydrogenase -
H7R04_RS08665 1831072..1831971 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R04_RS08670 1832117..1832167 - 51 WP_001364200.1 his operon leader peptide -
H7R04_RS08675 1832451..1832702 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R04_RS08680 1832699..1832953 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R04_RS08685 1833036..1833860 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R04_RS08690 1833906..1834835 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R04_RS08695 1835050..1835112 + 63 WP_010723108.1 membrane protein YoeI -
H7R04_RS08700 1835102..1836460 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R04_RS08705 1836639..1837697 + 1059 WP_000492330.1 transport protein YeeE -
H7R04_RS08710 1837711..1837938 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34621 WP_000767829.1 NZ_AP022120:1832699-1832953 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34621 NZ_AP022120:1832699-1832953 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34621 WP_001259255.1 NZ_AP022120:1832451-1832702 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34621 NZ_AP022120:1832451-1832702 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References