Detailed information of TA system TA034593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 81051..81682
• Replicon: plasmid pWP5-W18-ESBL-02_1
• Accession: NZ_AP022111
• Organism: Aeromonas caviae strain WP5-W18-ESBL-02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R67_RS22980
• Protein ID: WP_182959060.1
• Coordinates: 81353..81682 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R67_RS22975
• Protein ID: WP_182936404.1
• Coordinates: 81051..81353 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R67_RS22950	76887..77144	-	258	Protein_82	ISAs1 family transposase	-
H7R67_RS22955	77244..78497	+	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-
H7R67_RS22960	78529..78849	-	321	Protein_84	ISAs1 family transposase	-
H7R67_RS22965	78882..80135	-	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-
H7R67_RS22970	80235..80810	-	576	Protein_86	ISAs1 family transposase	-
H7R67_RS22975	81051..81353	-	303	WP_182936404.1	XRE family transcriptional regulator	Antitoxin
H7R67_RS22980	81353..81682	-	330	WP_182959060.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R67_RS22985	81702..82955	+	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-
H7R67_RS22990	83314..83928	-	615	WP_182936402.1	recombinase family protein	-
H7R67_RS22995	84079..85149	-	1071	Protein_91	IS21 family transposase	-
H7R67_RS23000	85312..86565	-	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..147986	147986
	-	inside	IScluster/Tn	-	-	58811..91967	33156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (27-99)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-89)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12223.86 Da
• Isoelectric Point: 8.9297

>T34593 WP_182959060.1 NZ_AP022111:c81682-81353 [Aeromonas caviae]
MNSLSGINQQDTPPSLYPPYTRNHHNSGGPSVGRPLVDTIKGSSFTNLKELRVQHQGEPLRAFFAFDPLRQAIVLCAGNK
GGNEKRFYKEMLPIAEAEYARHLAELEMR

Nucleotide

• Length: 330 bp

>T34593 NZ_AP022111:c81682-81353 [Aeromonas caviae]
TTGAATTCTTTGAGCGGAATCAATCAGCAGGATACGCCACCTTCTCTATATCCCCCATACACCAGAAATCACCATAACTC
AGGTGGCCCATCGGTAGGGCGCCCTTTGGTTGATACCATTAAGGGTTCCAGCTTTACGAACCTCAAAGAGCTGAGAGTTC
AGCACCAGGGAGAACCGCTGCGGGCCTTCTTCGCTTTTGATCCGTTACGACAAGCAATTGTGCTGTGTGCCGGCAATAAG
GGCGGTAACGAGAAGAGGTTTTACAAAGAAATGTTGCCGATTGCTGAGGCTGAATACGCCAGGCATTTAGCTGAATTGGA
GATGCGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11060.81 Da
• Isoelectric Point: 5.1768

>AT34593 WP_182936404.1 NZ_AP022111:c81353-81051 [Aeromonas caviae]
MGKTLTEMLASRSADSQQRIKDMADELMLEVRLQAVREELEVSQVELASALGITQPAIVAMEQRGSDVKLSTMKRYVEAM
GGKLRVDVELPTGRHIGFNV

Nucleotide

• Length: 303 bp

>AT34593 NZ_AP022111:c81353-81051 [Aeromonas caviae]
ATGGGAAAAACACTGACTGAAATGCTAGCCTCCCGCTCGGCGGATAGCCAGCAACGTATCAAGGATATGGCTGACGAGCT
GATGCTGGAAGTACGCTTGCAAGCTGTGCGCGAAGAGCTAGAGGTCTCCCAGGTGGAATTAGCCTCAGCACTCGGTATCA
CACAACCAGCTATTGTGGCGATGGAGCAGCGCGGTAGCGATGTCAAACTATCCACAATGAAGCGCTACGTGGAGGCTATG
GGTGGCAAGCTGCGTGTGGATGTAGAGCTTCCTACAGGACGACATATCGGCTTCAACGTTTGA