Detailed information of TA system TA034547

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96711..96965
• Replicon: plasmid pWP5-S17-ESBL-10_1
• Accession: NZ_AP022106
• Organism: Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H7R00_RS23975
• Protein ID: WP_015387399.1
• Coordinates: 96759..96965 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	96711..96772 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R00_RS23940	92363..92575	+	213	WP_005012601.1	hypothetical protein	-
H7R00_RS23945	92876..92965	-	90	Protein_108	IS1 family transposase	-
H7R00_RS23950	93020..93697	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
H7R00_RS23955	93697..94044	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R00_RS23960	94064..95635	+	1572	WP_023149734.1	IS66 family transposase	-
H7R00_RS23965	95673..96289	-	617	Protein_112	IS1 family transposase	-
H7R00_RS23970	96390..96572	+	183	WP_000968309.1	hypothetical protein	-
-	96711..96772	-	62	NuclAT_1	-	Antitoxin
-	96711..96772	-	62	NuclAT_1	-	Antitoxin
-	96711..96772	-	62	NuclAT_1	-	Antitoxin
-	96711..96772	-	62	NuclAT_1	-	Antitoxin
H7R00_RS23975	96759..96965	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R00_RS23980	97249..97506	+	258	WP_000083833.1	replication regulatory protein RepA	-
H7R00_RS23985	97742..97816	+	75	WP_032336874.1	RepA leader peptide Tap	-
H7R00_RS23990	97809..98255	+	447	Protein_117	incFII family plasmid replication initiator RepA	-
H7R00_RS23995	98201..98683	-	483	Protein_118	VENN motif pre-toxin domain-containing protein	-
H7R00_RS24000	99576..100796	+	1221	WP_000410951.1	arginine deiminase	-
H7R00_RS24005	100807..101718	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	iucA / iucB / iucC / iucD / iutA / senB	1..130869	130869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T34547 WP_015387399.1 NZ_AP022106:96759-96965 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T34547 NZ_AP022106:96759-96965 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT34547 NZ_AP022106:c96772-96711 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin