Detailed information of TA system TA034543

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56598..56868
• Replicon: plasmid pWP5-S17-ESBL-10_1
• Accession: NZ_AP022106
• Organism: Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R00_RS23745
• Protein ID: WP_001312861.1
• Coordinates: 56710..56868 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	56598..56661 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R00_RS23700	52706..53271	+	566	Protein_59	class I SAM-dependent methyltransferase	-
H7R00_RS23705	53297..53509	+	213	WP_001348622.1	hypothetical protein	-
H7R00_RS23710	53919..54134	+	216	WP_182912587.1	single-stranded DNA-binding protein	-
H7R00_RS23715	54160..54699	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
H7R00_RS23720	54767..55000	+	234	WP_000005990.1	DUF905 family protein	-
H7R00_RS23725	55028..55225	+	198	Protein_64	hypothetical protein	-
H7R00_RS23730	55280..55714	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
H7R00_RS23735	55711..56430	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
H7R00_RS23740	56442..56630	-	189	WP_001299721.1	hypothetical protein	-
-	56442..56666	+	225	NuclAT_0	-	-
-	56442..56666	+	225	NuclAT_0	-	-
-	56442..56666	+	225	NuclAT_0	-	-
-	56442..56666	+	225	NuclAT_0	-	-
-	56598..56661	-	64	-	-	Antitoxin
H7R00_RS23745	56710..56868	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R00_RS23750	57789..58076	+	288	WP_000107535.1	hypothetical protein	-
H7R00_RS23755	58194..59015	+	822	WP_001234426.1	DUF945 domain-containing protein	-
H7R00_RS23760	59312..59914	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
H7R00_RS23765	60235..60618	+	384	WP_001151524.1	relaxosome protein TraM	-
H7R00_RS23770	60805..61494	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	iucA / iucB / iucC / iucD / iutA / senB	1..130869	130869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T34543 WP_001312861.1 NZ_AP022106:56710-56868 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34543 NZ_AP022106:56710-56868 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT34543 NZ_AP022106:c56661-56598 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin