Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3796272..3796966 Replicon chromosome
Accession NZ_AP022105
Organism Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H7R00_RS18285 Protein ID WP_001263489.1
Coordinates 3796272..3796670 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H7R00_RS18290 Protein ID WP_000554758.1
Coordinates 3796673..3796966 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3791860..3791940 - 81 NuclAT_10 - -
- 3791860..3791940 - 81 NuclAT_10 - -
- 3791860..3791940 - 81 NuclAT_10 - -
- 3791860..3791940 - 81 NuclAT_10 - -
H7R00_RS18260 3792536..3792994 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H7R00_RS18265 3793255..3794712 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
H7R00_RS18270 3794769..3795290 - 522 Protein_3577 peptide chain release factor H -
H7R00_RS18275 3795286..3795492 - 207 Protein_3578 RtcB family protein -
H7R00_RS18280 3795810..3796262 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
H7R00_RS18285 3796272..3796670 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H7R00_RS18290 3796673..3796966 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H7R00_RS18295 3797018..3798073 - 1056 WP_001226164.1 DNA polymerase IV -
H7R00_RS18300 3798144..3798929 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H7R00_RS18305 3798901..3800613 + 1713 Protein_3584 flagellar biosynthesis protein FlhA -
H7R00_RS18310 3800837..3801334 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T34534 WP_001263489.1 NZ_AP022105:c3796670-3796272 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T34534 NZ_AP022105:c3796670-3796272 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT34534 WP_000554758.1 NZ_AP022105:c3796966-3796673 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT34534 NZ_AP022105:c3796966-3796673 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References