Detailed information of TA system TA034534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3796272..3796966
• Replicon: chromosome
• Accession: NZ_AP022105
• Organism: Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H7R00_RS18285
• Protein ID: WP_001263489.1
• Coordinates: 3796272..3796670 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R00_RS18290
• Protein ID: WP_000554758.1
• Coordinates: 3796673..3796966 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3791860..3791940	-	81	NuclAT_10	-	-
-	3791860..3791940	-	81	NuclAT_10	-	-
-	3791860..3791940	-	81	NuclAT_10	-	-
-	3791860..3791940	-	81	NuclAT_10	-	-
H7R00_RS18260	3792536..3792994	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R00_RS18265	3793255..3794712	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H7R00_RS18270	3794769..3795290	-	522	Protein_3577	peptide chain release factor H	-
H7R00_RS18275	3795286..3795492	-	207	Protein_3578	RtcB family protein	-
H7R00_RS18280	3795810..3796262	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H7R00_RS18285	3796272..3796670	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R00_RS18290	3796673..3796966	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R00_RS18295	3797018..3798073	-	1056	WP_001226164.1	DNA polymerase IV	-
H7R00_RS18300	3798144..3798929	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H7R00_RS18305	3798901..3800613	+	1713	Protein_3584	flagellar biosynthesis protein FlhA	-
H7R00_RS18310	3800837..3801334	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T34534 WP_001263489.1 NZ_AP022105:c3796670-3796272 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T34534 NZ_AP022105:c3796670-3796272 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT34534 WP_000554758.1 NZ_AP022105:c3796966-3796673 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT34534 NZ_AP022105:c3796966-3796673 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3