34522

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1811896..1812398 Replicon chromosome
Accession NZ_AP022105
Organism Escherichia coli strain WP5-S17-ESBL-10

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R00_RS08515 Protein ID WP_000767829.1
Coordinates 1812144..1812398 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R00_RS08510 Protein ID WP_001259255.1
Coordinates 1811896..1812147 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R00_RS08485 1807074..1808141 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R00_RS08490 1808141..1809211 - 1071 WP_001518029.1 histidinol-phosphate transaminase -
H7R00_RS08495 1809208..1810512 - 1305 WP_001367667.1 histidinol dehydrogenase -
H7R00_RS08500 1810518..1811417 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
H7R00_RS08505 1811563..1811613 - 51 WP_001364200.1 his operon leader peptide -
H7R00_RS08510 1811896..1812147 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R00_RS08515 1812144..1812398 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R00_RS08520 1812481..1813305 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R00_RS08525 1813351..1814280 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R00_RS08530 1814495..1814557 + 63 WP_010723108.1 membrane protein YoeI -
H7R00_RS08535 1814547..1815905 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R00_RS08540 1816084..1817142 + 1059 WP_000492333.1 transport protein YeeE -
H7R00_RS08545 1817156..1817383 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34522 WP_000767829.1 NZ_AP022105:1812144-1812398 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34522 NZ_AP022105:1812144-1812398 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34522 WP_001259255.1 NZ_AP022105:1811896-1812147 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34522 NZ_AP022105:1811896-1812147 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References