Detailed information of TA system TA034516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4923451..4924053
• Replicon: chromosome
• Accession: NZ_AP022098
• Organism: Escherichia coli strain WP4-S18-ESBL-09

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7R19_RS23595
• Protein ID: WP_000897305.1
• Coordinates: 4923742..4924053 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R19_RS23590
• Protein ID: WP_000356397.1
• Coordinates: 4923451..4923741 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R19_RS23570	4919953..4920855	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7R19_RS23575	4920852..4921487	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7R19_RS23580	4921484..4922413	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
H7R19_RS23585	4922628..4922846	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
H7R19_RS23590	4923451..4923741	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7R19_RS23595	4923742..4924053	-	312	WP_000897305.1	hypothetical protein	Toxin
H7R19_RS23600	4924282..4925190	+	909	WP_001318161.1	alpha/beta hydrolase	-
H7R19_RS23605	4925254..4926195	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R19_RS23610	4926240..4926677	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R19_RS23615	4926674..4927546	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H7R19_RS23620	4927540..4928139	-	600	WP_001314324.1	glucose-1-phosphatase	-
H7R19_RS23625	4928238..4929023	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T34516 WP_000897305.1 NZ_AP022098:c4924053-4923742 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T34516 NZ_AP022098:c4924053-4923742 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT34516 WP_000356397.1 NZ_AP022098:c4923741-4923451 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT34516 NZ_AP022098:c4923741-4923451 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7