Detailed information of TA system TA034488

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63822..64086
• Replicon: plasmid pWP4-S18-ESBL-08_2
• Accession: NZ_AP022097
• Organism: Escherichia coli strain WP4-S18-ESBL-08

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: H7R08_RS24820
• Protein ID: WP_001303307.1
• Coordinates: 63934..64086 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	63822..63884 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R08_RS24805	59061..61352	-	2292	WP_024255997.1	hypothetical protein	-
H7R08_RS24810	61345..62415	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
H7R08_RS24815	62434..63642	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	63822..63884	-	63	NuclAT_0	-	Antitoxin
-	63822..63884	-	63	NuclAT_0	-	Antitoxin
-	63822..63884	-	63	NuclAT_0	-	Antitoxin
-	63822..63884	-	63	NuclAT_0	-	Antitoxin
H7R08_RS24820	63934..64086	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
H7R08_RS24825	64158..64409	-	252	WP_001291965.1	hypothetical protein	-
-	64796..64847	-	52	NuclAT_1	-	-
-	64796..64847	-	52	NuclAT_1	-	-
-	64796..64847	-	52	NuclAT_1	-	-
-	64796..64847	-	52	NuclAT_1	-	-
H7R08_RS24830	65333..65509	-	177	WP_001054900.1	hypothetical protein	-
H7R08_RS24835	65901..66110	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H7R08_RS24840	66182..66844	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
H7R08_RS24845	66915..69083	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-8	-	1..93398	93398

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T34488 WP_001303307.1 NZ_AP022097:63934-64086 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T34488 NZ_AP022097:63934-64086 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT34488 NZ_AP022097:c63884-63822 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin