Detailed information of TA system TA034474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3795148..3795842
• Replicon: chromosome
• Accession: NZ_AP022095
• Organism: Escherichia coli strain WP4-S18-ESBL-08

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: H7R08_RS18420
• Protein ID: WP_001263493.1
• Coordinates: 3795148..3795546 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: H7R08_RS18425
• Protein ID: WP_000554757.1
• Coordinates: 3795549..3795842 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R08_RS18390	3790148..3791392	-	1245	WP_000189541.1	esterase FrsA	-
-	3790808..3790888	-	81	NuclAT_12	-	-
-	3790808..3790888	-	81	NuclAT_12	-	-
-	3790808..3790888	-	81	NuclAT_12	-	-
-	3790808..3790888	-	81	NuclAT_12	-	-
H7R08_RS18395	3791484..3791942	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R08_RS18400	3792203..3793660	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
H7R08_RS18405	3793717..3794238	-	522	Protein_3606	peptide chain release factor H	-
H7R08_RS18410	3794237..3794440	-	204	Protein_3607	RNA ligase RtcB family protein	-
H7R08_RS18415	3794686..3795138	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
H7R08_RS18420	3795148..3795546	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R08_RS18425	3795549..3795842	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R08_RS18430	3795894..3796949	-	1056	WP_001226164.1	DNA polymerase IV	-
H7R08_RS18435	3797020..3797805	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
H7R08_RS18440	3797777..3799489	+	1713	Protein_3613	flagellar biosynthesis protein FlhA	-
H7R08_RS18445	3799705..3800202	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T34474 WP_001263493.1 NZ_AP022095:c3795546-3795148 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T34474 NZ_AP022095:c3795546-3795148 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT34474 WP_000554757.1 NZ_AP022095:c3795842-3795549 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT34474 NZ_AP022095:c3795842-3795549 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6