Detailed information of TA system TA034450

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96382..96797
• Replicon: plasmid pWP4-S18-ESBL-07_1
• Accession: NZ_AP022088
• Organism: Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: H7R53_RS23990
• Protein ID: WP_000775238.1
• Coordinates: 96382..96543 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	96589..96797 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R53_RS23970	92455..92955	-	501	WP_085326112.1	antirestriction protein ArdA	-
H7R53_RS26105	93418..93615	-	198	Protein_93	hypothetical protein	-
H7R53_RS23975	93695..94099	-	405	WP_001175008.1	IS200/IS605 family transposase	-
H7R53_RS23980	94158..95384	+	1227	WP_182920558.1	transposase	-
H7R53_RS23985	95684..96187	+	504	WP_085326126.1	GNAT family N-acetyltransferase	-
H7R53_RS23990	96382..96543	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	96589..96797	-	209	NuclAT_0	-	Antitoxin
-	96589..96797	-	209	NuclAT_0	-	Antitoxin
-	96589..96797	-	209	NuclAT_0	-	Antitoxin
-	96589..96797	-	209	NuclAT_0	-	Antitoxin
H7R53_RS23995	96809..97528	-	720	WP_072645262.1	plasmid SOS inhibition protein A	-
H7R53_RS24000	97525..97959	-	435	WP_085326114.1	conjugation system SOS inhibitor PsiB	-
H7R53_RS24005	98014..99972	-	1959	WP_182920546.1	ParB/RepB/Spo0J family partition protein	-
H7R53_RS24010	100036..100269	-	234	WP_021564200.1	DUF905 family protein	-
H7R53_RS24015	100327..100854	-	528	WP_001276114.1	single-stranded DNA-binding protein	-
H7R53_RS24020	101223..101291	+	69	Protein_103	pilus protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..183772	183772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T34450 WP_000775238.1 NZ_AP022088:c96543-96382 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T34450 NZ_AP022088:c96543-96382 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 209 bp

>AT34450 NZ_AP022088:c96797-96589 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAAGCGCGCAGGGATATGGGCTATGA
TGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGACAAAAGCC
CCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin