Detailed information of TA system TA034448

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75824..76088
• Replicon: plasmid pWP4-S18-ESBL-07_1
• Accession: NZ_AP022088
• Organism: Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: H7R53_RS23880
• Protein ID: WP_001331364.1
• Coordinates: 75824..75976 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	76026..76088 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R53_RS23855	71009..73177	+	2169	WP_182920555.1	DotA/TraY family protein	-
H7R53_RS23860	73242..73904	+	663	WP_001542510.1	plasmid IncI1-type surface exclusion protein ExcA	-
H7R53_RS23865	74002..74211	+	210	WP_001140544.1	hemolysin expression modulator Hha	-
H7R53_RS23870	74420..74596	+	177	WP_001054905.1	hypothetical protein	-
H7R53_RS23875	75501..75752	+	252	WP_001618083.1	hypothetical protein	-
H7R53_RS23880	75824..75976	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	76026..76088	+	63	NuclAT_1	-	Antitoxin
-	76026..76088	+	63	NuclAT_1	-	Antitoxin
-	76026..76088	+	63	NuclAT_1	-	Antitoxin
-	76026..76088	+	63	NuclAT_1	-	Antitoxin
H7R53_RS23885	76268..77476	+	1209	WP_182920556.1	IncI1-type conjugal transfer protein TrbA	-
H7R53_RS23890	77495..78565	+	1071	WP_000151586.1	IncI1-type conjugal transfer protein TrbB	-
H7R53_RS23895	78558..80849	+	2292	WP_085326102.1	conjugal transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..183772	183772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T34448 WP_001331364.1 NZ_AP022088:c75976-75824 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T34448 NZ_AP022088:c75976-75824 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT34448 NZ_AP022088:76026-76088 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin