Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4794977..4795579 Replicon chromosome
Accession NZ_AP022087
Organism Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag H7R53_RS22575 Protein ID WP_024046152.1
Coordinates 4795268..4795579 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H7R53_RS22570 Protein ID WP_000356395.1
Coordinates 4794977..4795267 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R53_RS22535 4790601..4791503 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
H7R53_RS22540 4791500..4792135 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
H7R53_RS22545 4792132..4793061 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
H7R53_RS22550 4793243..4793485 - 243 WP_001309881.1 ribbon-helix-helix domain-containing protein -
H7R53_RS22555 4793704..4793922 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
H7R53_RS22560 4794341..4794619 - 279 WP_001315112.1 hypothetical protein -
H7R53_RS22565 4794671..4794892 - 222 WP_001550354.1 hypothetical protein -
H7R53_RS22570 4794977..4795267 - 291 WP_000356395.1 helix-turn-helix domain-containing protein Antitoxin
H7R53_RS22575 4795268..4795579 - 312 WP_024046152.1 hypothetical protein Toxin
H7R53_RS22580 4795808..4796716 + 909 WP_001550353.1 alpha/beta hydrolase -
H7R53_RS22585 4796884..4798698 - 1815 WP_024046153.1 hypothetical protein -
H7R53_RS22590 4799114..4800055 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
H7R53_RS22595 4800100..4800537 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12130.16 Da        Isoelectric Point: 9.5486

>T34445 WP_024046152.1 NZ_AP022087:c4795579-4795268 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHCEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T34445 NZ_AP022087:c4795579-4795268 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTGTGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT34445 WP_000356395.1 NZ_AP022087:c4795267-4794977 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT34445 NZ_AP022087:c4795267-4794977 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References