Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1900921..1901423 Replicon chromosome
Accession NZ_AP022087
Organism Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag H7R53_RS08945 Protein ID WP_000767820.1
Coordinates 1901169..1901423 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R53_RS08940 Protein ID WP_001259255.1
Coordinates 1900921..1901172 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R53_RS08915 1896099..1897166 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R53_RS08920 1897166..1898236 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
H7R53_RS08925 1898233..1899537 - 1305 WP_001578572.1 histidinol dehydrogenase -
H7R53_RS08930 1899543..1900442 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R53_RS08935 1900588..1900638 - 51 WP_001364200.1 his operon leader peptide -
H7R53_RS08940 1900921..1901172 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R53_RS08945 1901169..1901423 + 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R53_RS08950 1901506..1902330 + 825 WP_000754756.1 SDR family oxidoreductase -
H7R53_RS08955 1902376..1903305 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R53_RS08960 1903520..1903582 + 63 WP_010723108.1 membrane protein YoeI -
H7R53_RS08965 1903572..1904930 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R53_RS08970 1905109..1906167 + 1059 WP_001759318.1 transport protein YeeE -
H7R53_RS08975 1906181..1906408 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T34426 WP_000767820.1 NZ_AP022087:1901169-1901423 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34426 NZ_AP022087:1901169-1901423 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34426 WP_001259255.1 NZ_AP022087:1900921-1901172 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34426 NZ_AP022087:1900921-1901172 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References