Detailed information of TA system TA034389

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52602..52855
• Replicon: plasmid pWP4-W18-ESBL-09_3
• Accession: NZ_AP022072
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H7R28_RS26705
• Protein ID: WP_001312851.1
• Coordinates: 52706..52855 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52602..52661 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS26670	48378..49238	+	861	WP_000704524.1	alpha/beta hydrolase	-
H7R28_RS26675	49341..49901	+	561	WP_000139321.1	fertility inhibition protein FinO	-
H7R28_RS26680	50030..50242	+	213	WP_001309245.1	ANR family transcriptional regulator	-
H7R28_RS26685	50487..50948	+	462	WP_016230918.1	thermonuclease family protein	-
H7R28_RS26690	50994..51203	+	210	WP_001369435.1	hemolysin expression modulator Hha	-
H7R28_RS26695	51241..51831	+	591	WP_042018152.1	DUF2726 domain-containing protein	-
H7R28_RS26700	51986..52459	+	474	WP_016240489.1	hypothetical protein	-
-	52602..52661	-	60	NuclAT_1	-	Antitoxin
-	52602..52661	-	60	NuclAT_1	-	Antitoxin
-	52602..52661	-	60	NuclAT_1	-	Antitoxin
-	52602..52661	-	60	NuclAT_1	-	Antitoxin
H7R28_RS26705	52706..52855	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R28_RS26710	53140..53388	+	249	WP_000083839.1	replication regulatory protein RepA	-
H7R28_RS26715	53633..53707	+	75	WP_001365705.1	RepA leader peptide Tap	-
H7R28_RS26720	53700..54557	+	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
H7R28_RS26725	55472..55741	+	270	WP_000079928.1	hypothetical protein	-
H7R28_RS26730	55738..56019	+	282	WP_000970000.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H7R28_RS26735	56065..56913	+	849	WP_032256585.1	3'-5' exonuclease	-
H7R28_RS26740	57030..57512	+	483	WP_001311056.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..77891	77891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T34389 WP_001312851.1 NZ_AP022072:52706-52855 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T34389 NZ_AP022072:52706-52855 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT34389 NZ_AP022072:c52661-52602 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin