Detailed information of TA system TA034385

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 10061..10331
• Replicon: plasmid pWP4-W18-ESBL-09_3
• Accession: NZ_AP022072
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R28_RS26440
• Protein ID: WP_001312861.1
• Coordinates: 10173..10331 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	10061..10124 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS26405	5086..5529	-	444	WP_128472901.1	hypothetical protein	-
H7R28_RS26410	5831..6358	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H7R28_RS26415	6416..6649	+	234	WP_033551487.1	DUF905 family protein	-
H7R28_RS26420	6710..8674	+	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
H7R28_RS26425	8743..9177	+	435	WP_052983109.1	conjugation system SOS inhibitor PsiB	-
H7R28_RS26430	9174..9893	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	9905..10129	+	225	NuclAT_0	-	-
-	9905..10129	+	225	NuclAT_0	-	-
-	9905..10129	+	225	NuclAT_0	-	-
-	9905..10129	+	225	NuclAT_0	-	-
H7R28_RS26435	9914..10093	-	180	WP_001309233.1	hypothetical protein	-
-	10061..10124	-	64	-	-	Antitoxin
H7R28_RS26440	10173..10331	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R28_RS26445	10632..10836	-	205	Protein_14	pilus protein	-
H7R28_RS26450	11228..11524	+	297	WP_001272251.1	hypothetical protein	-
H7R28_RS26455	11635..12456	+	822	WP_001234445.1	DUF945 domain-containing protein	-
H7R28_RS26460	12753..13343	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
H7R28_RS26465	13676..14059	+	384	WP_001151529.1	relaxosome protein TraM	-
H7R28_RS26470	14251..14898	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
H7R28_RS26475	15006..15245	+	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..77891	77891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T34385 WP_001312861.1 NZ_AP022072:10173-10331 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34385 NZ_AP022072:10173-10331 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGCCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT34385 NZ_AP022072:c10124-10061 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin