Detailed information of TA system TA034382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5085129..5085731
• Replicon: chromosome
• Accession: NZ_AP022069
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7R28_RS24270
• Protein ID: WP_000897305.1
• Coordinates: 5085420..5085731 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R28_RS24265
• Protein ID: WP_000356395.1
• Coordinates: 5085129..5085419 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS24230	5080752..5081654	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7R28_RS24235	5081651..5082286	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7R28_RS24240	5082283..5083212	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H7R28_RS24245	5083394..5083636	-	243	WP_021523315.1	hypothetical protein	-
H7R28_RS24250	5083855..5084073	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H7R28_RS24255	5084492..5084770	-	279	WP_001306650.1	hypothetical protein	-
H7R28_RS24260	5084832..5085044	-	213	WP_000197774.1	hypothetical protein	-
H7R28_RS24265	5085129..5085419	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
H7R28_RS24270	5085420..5085731	-	312	WP_000897305.1	hypothetical protein	Toxin
H7R28_RS24275	5085960..5086868	+	909	WP_001306651.1	alpha/beta hydrolase	-
H7R28_RS24280	5087037..5088851	-	1815	WP_020233236.1	hypothetical protein	-
H7R28_RS24285	5089267..5090208	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R28_RS24290	5090253..5090690	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T34382 WP_000897305.1 NZ_AP022069:c5085731-5085420 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T34382 NZ_AP022069:c5085731-5085420 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT34382 WP_000356395.1 NZ_AP022069:c5085419-5085129 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT34382 NZ_AP022069:c5085419-5085129 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7