Detailed information of TA system TA034374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4065845..4066539
• Replicon: chromosome
• Accession: NZ_AP022069
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: H7R28_RS19435
• Protein ID: WP_001521903.1
• Coordinates: 4065845..4066243 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R28_RS19440
• Protein ID: WP_000554758.1
• Coordinates: 4066246..4066539 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS19405	4061015..4062259	-	1245	WP_000189541.1	esterase FrsA	-
-	4061675..4061755	-	81	NuclAT_11	-	-
-	4061675..4061755	-	81	NuclAT_11	-	-
-	4061675..4061755	-	81	NuclAT_11	-	-
-	4061675..4061755	-	81	NuclAT_11	-	-
H7R28_RS19410	4062351..4062809	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R28_RS19415	4063070..4064527	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
H7R28_RS27015	4064584..4064936	-	353	Protein_3799	peptide chain release factor H	-
H7R28_RS19425	4064932..4065138	-	207	Protein_3800	RtcB family protein	-
H7R28_RS19430	4065383..4065835	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
H7R28_RS19435	4065845..4066243	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R28_RS19440	4066246..4066539	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R28_RS19445	4066591..4067646	-	1056	WP_001226177.1	DNA polymerase IV	-
H7R28_RS19450	4067717..4068502	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
H7R28_RS19455	4068474..4070186	+	1713	Protein_3806	flagellar biosynthesis protein FlhA	-
H7R28_RS19460	4070265..4070618	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
H7R28_RS19465	4070675..4071424	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4055782..4066539	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T34374 WP_001521903.1 NZ_AP022069:c4066243-4065845 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T34374 NZ_AP022069:c4066243-4065845 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT34374 WP_000554758.1 NZ_AP022069:c4066539-4066246 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT34374 NZ_AP022069:c4066539-4066246 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3