Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1970614..1971116 Replicon chromosome
Accession NZ_AP022069
Organism Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R28_RS09150 Protein ID WP_000767829.1
Coordinates 1970862..1971116 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R28_RS09145 Protein ID WP_001259255.1
Coordinates 1970614..1970865 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R28_RS09120 1965792..1966859 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R28_RS09125 1966859..1967929 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
H7R28_RS09130 1967926..1969230 - 1305 WP_021523144.1 histidinol dehydrogenase -
H7R28_RS09135 1969236..1970135 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
H7R28_RS09140 1970281..1970331 - 51 WP_001364200.1 his operon leader peptide -
H7R28_RS09145 1970614..1970865 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R28_RS09150 1970862..1971116 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R28_RS09155 1971199..1972023 + 825 WP_000754758.1 SDR family oxidoreductase -
H7R28_RS09160 1972069..1972998 + 930 WP_023154044.1 LysR family transcriptional regulator -
H7R28_RS09165 1973213..1973275 + 63 WP_010723108.1 membrane protein YoeI -
H7R28_RS09170 1973265..1974623 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R28_RS09175 1974802..1975860 + 1059 WP_000492324.1 transport protein YeeE -
H7R28_RS09180 1975874..1976101 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34358 WP_000767829.1 NZ_AP022069:1970862-1971116 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34358 NZ_AP022069:1970862-1971116 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34358 WP_001259255.1 NZ_AP022069:1970614-1970865 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34358 NZ_AP022069:1970614-1970865 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References