Detailed information of TA system TA034357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1234673..1235400
• Replicon: chromosome
• Accession: NZ_AP022069
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: H7R28_RS05825
• Protein ID: WP_001521139.1
• Coordinates: 1234673..1234984 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R28_RS05830
• Protein ID: WP_000126294.1
• Coordinates: 1234981..1235400 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS05800	1230608..1232317	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
H7R28_RS05805	1232327..1232869	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
H7R28_RS05810	1232869..1233636	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
H7R28_RS05815	1233633..1234043	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
H7R28_RS05820	1234036..1234506	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
H7R28_RS05825	1234673..1234984	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R28_RS05830	1234981..1235400	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
H7R28_RS05835	1235479..1236903	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
H7R28_RS05840	1236912..1238369	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
H7R28_RS05845	1238629..1239639	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
H7R28_RS05850	1239788..1240315	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T34357 WP_001521139.1 NZ_AP022069:1234673-1234984 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T34357 NZ_AP022069:1234673-1234984 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT34357 WP_000126294.1 NZ_AP022069:1234981-1235400 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT34357 NZ_AP022069:1234981-1235400 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9