Detailed information of TA system TA034347

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44274..44538
• Replicon: plasmid pWP4-W18-ESBL-08_2
• Accession: NZ_AP022066
• Organism: Escherichia coli strain WP4-W18-ESBL-08

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: H7R03_RS24915
• Protein ID: WP_001331364.1
• Coordinates: 44274..44426 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	44476..44538 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R03_RS24890	39551..41719	+	2169	WP_021537350.1	DotA/TraY family protein	-
H7R03_RS24895	41793..42443	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
H7R03_RS24900	42515..42724	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H7R03_RS24905	43116..43292	+	177	WP_001054904.1	hypothetical protein	-
H7R03_RS24910	43951..44202	+	252	WP_001291964.1	hypothetical protein	-
H7R03_RS24915	44274..44426	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	44476..44538	+	63	NuclAT_0	-	Antitoxin
-	44476..44538	+	63	NuclAT_0	-	Antitoxin
-	44476..44538	+	63	NuclAT_0	-	Antitoxin
-	44476..44538	+	63	NuclAT_0	-	Antitoxin
H7R03_RS24920	44718..45926	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
H7R03_RS24925	45945..47015	+	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
H7R03_RS24930	47008..49299	+	2292	WP_001289270.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..95805	95805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T34347 WP_001331364.1 NZ_AP022066:c44426-44274 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T34347 NZ_AP022066:c44426-44274 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT34347 NZ_AP022066:44476-44538 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin