Detailed information of TA system TA034339

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64121..64363
• Replicon: plasmid pWP4-W18-ESBL-08_1
• Accession: NZ_AP022065
• Organism: Escherichia coli strain WP4-W18-ESBL-08

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R03_RS24245
• Protein ID: WP_001312861.1
• Coordinates: 64205..64363 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	64121..64161 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R03_RS24225	59316..61274	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
H7R03_RS24230	61329..61763	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
H7R03_RS24235	61760..62479	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	62491..62677	+	187	NuclAT_0	-	-
-	62491..62677	+	187	NuclAT_0	-	-
-	62491..62677	+	187	NuclAT_0	-	-
-	62491..62677	+	187	NuclAT_0	-	-
H7R03_RS24240	62725..64094	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	64121..64161	+	41	NuclAT_1	-	Antitoxin
-	64121..64161	+	41	NuclAT_1	-	Antitoxin
-	64121..64161	+	41	NuclAT_1	-	Antitoxin
-	64121..64161	+	41	NuclAT_1	-	Antitoxin
H7R03_RS24245	64205..64363	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R03_RS24250	64806..65141	+	336	WP_013023876.1	hypothetical protein	-
H7R03_RS24255	65054..65260	+	207	WP_000275859.1	hypothetical protein	-
H7R03_RS24260	65285..65572	+	288	WP_000107535.1	hypothetical protein	-
H7R03_RS24265	65691..66512	+	822	WP_001234469.1	DUF945 domain-containing protein	-
H7R03_RS24270	66809..67411	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H7R03_RS24275	67742..68125	+	384	WP_001151566.1	relaxosome protein TraM	-
H7R03_RS24280	68259..68936	+	678	WP_001348626.1	PAS domain-containing protein	-
H7R03_RS24285	69024..69251	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..134864	134864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T34339 WP_001312861.1 NZ_AP022065:64205-64363 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34339 NZ_AP022065:64205-64363 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT34339 NZ_AP022065:64121-64161 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin