Detailed information of TA system TA034336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4758825..4759427
• Replicon: chromosome
• Accession: NZ_AP022064
• Organism: Escherichia coli strain WP4-W18-ESBL-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: H7R03_RS22875
• Protein ID: WP_000897302.1
• Coordinates: 4759116..4759427 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R03_RS22870
• Protein ID: WP_000356397.1
• Coordinates: 4758825..4759115 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R03_RS22840	4754132..4755061	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
H7R03_RS22845	4755243..4755485	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
H7R03_RS22850	4755775..4756623	+	849	WP_001038650.1	hypothetical protein	-
H7R03_RS22855	4756939..4757388	+	450	WP_032140890.1	hypothetical protein	-
H7R03_RS22860	4757573..4757791	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
H7R03_RS22865	4758188..4758466	-	279	WP_001296612.1	hypothetical protein	-
H7R03_RS22870	4758825..4759115	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7R03_RS22875	4759116..4759427	-	312	WP_000897302.1	hypothetical protein	Toxin
H7R03_RS22880	4759656..4760564	+	909	WP_001385591.1	alpha/beta hydrolase	-
H7R03_RS22885	4760628..4761569	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R03_RS22890	4761614..4762051	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R03_RS22895	4762048..4762920	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H7R03_RS22900	4762914..4763513	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T34336 WP_000897302.1 NZ_AP022064:c4759427-4759116 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T34336 NZ_AP022064:c4759427-4759116 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT34336 WP_000356397.1 NZ_AP022064:c4759115-4758825 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT34336 NZ_AP022064:c4759115-4758825 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416