Detailed information of TA system TA034288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 697883..698619
• Replicon: chromosome
• Accession: NZ_AP022057
• Organism: Klebsiella sp. WP4-W18-ESBL-05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R58_RS03425
• Protein ID: WP_182929979.1
• Coordinates: 697883..698200 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R58_RS03430
• Protein ID: WP_181358007.1
• Coordinates: 698203..698619 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R58_RS03405	693651..694619	-	969	WP_106927107.1	TerC family protein	-
H7R58_RS03410	694892..695878	-	987	WP_182929977.1	Gfo/Idh/MocA family oxidoreductase	-
H7R58_RS03415	695951..696451	-	501	WP_106927103.1	M48 family metallopeptidase	-
H7R58_RS03420	696535..697671	+	1137	WP_182929978.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
H7R58_RS03425	697883..698200	+	318	WP_182929979.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R58_RS03430	698203..698619	+	417	WP_181358007.1	helix-turn-helix domain-containing protein	Antitoxin
H7R58_RS03435	698623..700641	-	2019	WP_182929980.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
H7R58_RS03440	700779..701861	-	1083	WP_182929981.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
H7R58_RS03445	701858..703246	-	1389	WP_182934592.1	phosphomannomutase CpsG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-136)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12235.23 Da
• Isoelectric Point: 10.0532

>T34288 WP_182929979.1 NZ_AP022057:697883-698200 [Klebsiella sp. WP4-W18-ESBL-05]
MHLISMAAIDEGCGRFPQFAKEIKLLGKVIAKANCPTPEALRAFYPSLDNFKYLDKHYVVNIANNNIRVIALIFFESQKF
YIRHIFNHAEYDRFTDKHRTKGKKS

Nucleotide

• Length: 318 bp

>T34288 NZ_AP022057:697883-698200 [Klebsiella sp. WP4-W18-ESBL-05]
ATGCATCTGATATCAATGGCTGCAATTGACGAGGGATGTGGCAGATTTCCCCAGTTTGCGAAAGAAATTAAGCTGTTAGG
TAAGGTGATTGCGAAAGCAAACTGTCCAACGCCAGAAGCGTTGAGGGCATTTTATCCCTCATTGGATAACTTCAAATATC
TTGATAAACACTATGTTGTGAACATTGCTAACAATAATATCAGGGTGATTGCGTTGATTTTTTTCGAAAGCCAGAAGTTT
TATATCCGGCACATTTTTAACCACGCTGAGTACGATAGGTTCACAGATAAACACAGAACGAAGGGGAAGAAATCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15315.90 Da
• Isoelectric Point: 5.6982

>AT34288 WP_181358007.1 NZ_AP022057:698203-698619 [Klebsiella sp. WP4-W18-ESBL-05]
MMTAKALKATKTLVAEIPLLGDNPTEADHRDALELLAELLMDDPTNPLVDLLCVRIRAYEDNQPKLRALREEIEAIPTGI
AILRTLMEQHKLTLSDFADEIGSKSMVSRVLSGQRKLTLDHVKKLAARFGISPALFID

Nucleotide

• Length: 417 bp

>AT34288 NZ_AP022057:698203-698619 [Klebsiella sp. WP4-W18-ESBL-05]
ATGATGACGGCAAAAGCCCTGAAAGCCACAAAAACGCTGGTTGCTGAAATTCCTCTTCTGGGAGATAACCCCACAGAGGC
CGATCATCGTGACGCACTGGAACTGCTGGCGGAACTGCTGATGGACGACCCAACAAATCCGTTGGTTGATCTACTGTGCG
TTCGCATCCGTGCGTATGAGGACAACCAGCCGAAATTACGTGCGTTACGTGAAGAGATTGAGGCTATTCCCACGGGCATC
GCGATTTTGCGAACCCTGATGGAGCAGCATAAACTGACGCTTTCCGATTTTGCCGATGAGATTGGCAGCAAGTCGATGGT
TTCACGCGTACTCAGCGGTCAAAGAAAATTAACGCTGGATCATGTCAAAAAGCTGGCCGCTCGATTTGGCATTTCACCCG
CGTTATTTATTGATTAA